By Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek
The seriously acclaimed laboratory regular, Methods in Enzymology, is likely one of the such a lot hugely revered courses within the box of biochemistry. on account that 1955, every one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. The sequence includes a lot fabric nonetheless correct at the present time - actually a necessary ebook for researchers in all fields of lifestyles sciences
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Additional resources for Affinity labeling
Am. Chem. Soc. 98, 6762 (1976). ~oR. R. Rando, manuscript in preparation. I/+ I CH2 I \ H /---o / H (5) NH I CH:~ I I1+ I "-~N~i°~ H becomes progressively and irreversibly inactivated. 11 However, inhibition can be completely prevented by adding an exogenous mercaptan, such as fl-mercaptoethanol. We have here, then, an interesting example, in which the highly reactive molecule, once formed, diffuses into solution more rapidly than it reacts covalently with an active-site residue. Thus the acrolein concentration increases to inhibit the enzyme, but only as an affinity label.
Assoc. Cancer Res. 8, 25 (1967). See also this volume . 36 GENERAL METHODOLOGY ~] DONV does not affect this enzyme. This is as expected, since DONV should not be a substrate for the enzyme. Both azaserine and DON irreversibly inhibit formylglycinamide ribotide amidotransferase. 12 Again, enzymic protonation precedes inactivation. In general, acid proteases seem to be quite sensitive to inhibition by diazo compounds, although cupric ion may be required for a facile reaction. 1~ Recently, fl-galactosidase has been reported to be irreversibly inhibited by the diazo sugar 2,6-anhydro-l-diazo-l-deoxy-D-glycero-L-manno-heptitel.
Koetfler and G. P. Hess, Biochemistry 18, 5345 (1974). K. A. Koehler and G. E. Lienhard, Biochemistry 10, 2477 (1971). R. N. Lindquist and C. Terry, Arch. Biochem. Biophys. 160, 135 (1974). ° J. O. Westerik and R. Wolfenden, J. Biol. Chem. 247, 8195 (1971). P R. C. Thompson, Biochemistry 12, 47 (1973). J. O. Westerik a n d R . Wolfenden, J. Biol. Chem. 249, 6351 (1974). r j. D. Findlater and B. A. Orsi, FEBS Left. $6, 109 (1973) ° J. Conchie, A. J. Hay, I. Strachan, and G. A. Levvy, Biochem. J.
Affinity labeling by Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek