Read e-book online Automated Enzyme Assays PDF

By D.B. Roodyn (Eds.)

ISBN-10: 0080887732

ISBN-13: 9780080887739

ISBN-10: 0720442060

ISBN-13: 9780720442069

Within the face of fast advancements in automatic suggestions, this ebook offers a good consultant to offer traits. It offers info on: ideas and terminology of enzyme automation; computerized equipment illustrated by means of the Technicon procedure; semi-automatic equipment; interrupted-flow and discrete-sampling structures; single-enzyme research; multiple-enzyme research (M.E.A.); enzyme characterization; calculation of enzyme actions from tool readings; generalized platforms for enzyme automation; Appendices I. released automatic enzyme assays, II. Terminology utilized in enzyme automation, III. gear utilized in enzyme automation, IV. laptop software for generalized enzyme automatic process.

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Extra resources for Automated Enzyme Assays

Example text

The plot includes a trace in which the rate of oxygen uptake is obtained by a simple electronic circuit that differentiates the signal from the oxygen electrode. With the availability of multichannel recorders and X-Y plotters, further development of such ‘multi-parameter’ sensor systems will be facilitated. By providing simultaneous measurement of different properties of the reaction mixture, more meaningful data may be obtained than from sequential measurements. For certain enzymes, particularly the flavoproteins and cytochromes, direct measurement of the spectrum is extremely useful.

218 40 AUTOMATED ENZYME ASSAYS ferricyanide absorbs strongly at 420 nm and none of the other reactants or products, including ferrocyanide, absorb strongly at this wavelength. In order to avoid complications due to the need for deproteinization, I will consider the simplest case in which a soluble extract is used. 1. The manual method The manual assay is as follows. 4 mM K&(CN),. Enzyme is added to give a final concentration of 50 pg protein/ml, and after 10 min at 30 "C the fall in absorbance at 420 nm is measured.

The volume of fluid pumped by each tube, or line is determined by the internal diameter of the pump tubing. 1) where LV is flow rate or line volume in ml/min. Since many different TABLE3. I Pumping rates with Technicon tubing. (Values refer to clear standard tubing used with a standard-speed proportioning pump. Pumping rates should not fall below the minimum value or rise above the maximum value. 05 THE TECHNICON SYSTEM 31 diameters of tubing are available, a wide range of volumes may be delivered at one pumping speed.

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Automated Enzyme Assays by D.B. Roodyn (Eds.)


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