New PDF release: Ciba Foundation Symposium - Isotopes in Biochemistry

ISBN-10: 0470714689

ISBN-13: 9780470714683

ISBN-10: 0470715162

ISBN-13: 9780470715161

Chapter 1 Chairman's starting feedback (pages 1–3): A. S. McFarlane
Chapter 2 Metabolism of 14C?Labelled Steroids (pages 4–13): C. P. Leblond
Chapter three excessive ldl cholesterol content material of Human Spleen (pages 14–16): Karl Bernhard
Chapter four The Biosynthesis of Radioactive ldl cholesterol through Surviving Liver Slices (pages 17–27): Samuel Gurin and Roscoe O. Brady
Chapter five reports with Deuterium Steroids (pages 28–40): T. F. Gallagher
Chapter 6 The Biosynthetic Mechanism of Porphyrin Formation (pages 41–67): David Shemin and Jonathan Wittenberg
Chapter 7 reports on Mammalian purple Cells (pages 68–85): A. Neubergeb
Chapter eight initial Investigations for a learn of strength used by the Surviving bird Erythrocyte in H?m Synthesis (pages 86–89): C. Rimington
Chapter nine Iron Metabolism in Pathological stipulations (pages 90–95): A. Vannotti
Chapter 10 The amendment of X?Ray Sensitivity by way of chemical substances (pages 96–113): Alexander Hollaender, G. E. Stapleton and W. T. Burnett
Chapter eleven impact of X?Rays on Nucleic Acid and Protein Synthesis within the Jensen Rat Sarcoma (pages 114–121): Barbara E. Holmes
Chapter 12 Radiation Dose in Tracer Experiments concerning Autoradiography (pages 122–137): S. R. Pelc
Chapter thirteen Synthesis of Deoxyribose Nucleic Acid and Nuclear Incorporation of 35S as proven via Autoradiographs (pages 138–151): Alma Howard and S. R. Pelc
Chapter 14 The Biosynthesis of Pyrimidines in vitro (pages 152–163): D. Wright Wilson
Chapter 15 reports with natural? and Bio?Synthetic Nucleosides and Nucleotides (pages 164–174): G. B. Brown
Chapter sixteen using Radiophosphorus within the examine of the Nucleic Acids (pages 175–183): J. N. Davidson
Chapter 17 price of Synthesis and Quantitative diversifications of the Ribonucleic Acid through the development of a tradition of Polytomella Coeca (pages 184–189): R. Jeener
Chapter 18 a style for the evaluate of the speed of Protein Synthesis in guy (pages 190–202): D. Rittenberg
Chapter 19 Turnover premiums in the course of Formation of Proteins and Polynucleotides in Regenerating Tissues (pages 203–212): E. Hammarsten
Chapter 20 Synthesis of Phenylalanine and Tyrosine in Yeast (pages 213–226): Konrad Bloch
Chapter 21 A learn of Acetone Metabolism utilizing Glycogen and Serine as symptoms, and the function of C1?Compounds in Metabolism (pages 227–245): Harland G. Wood
Chapter 22 uneven Citric Acid (pages 246–257): Charles Heidelberger and Van R. Potter
Chapter 23 Mode of Formation of Fatty Acids from Acetate and Glucose as Studied within the Mammary Gland (pages 258–284): G. Popjak

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Subtract “endogenou~”products Isotopic metabolites recovered ... ...... ...... ...... Total ...... ...... ...... ...... ...... ...... 08 The values for the quantitatively minor ketonic metabolites were obtained by isotopic dilution alone since the amounts were too small t o permit direct isolation. In these instances, 38 T. F. GALLAGHER the endogenous production can be disregarded without error since it is very small in comparison with the amount of carrier added. The values obtained are shown in Table I.

Biol. , 162, 441. , and RITTENBERG, D. (1949). J . biol. , 179, 843. BOREK, BFLADY,R. , and GURIN,S. J . biol. , 186,461. BRADY,R. , and GURIN,S. (1950b). J . biol. , 187, 589. 24 SAMUELGURINAND ROSCOE0. BRADY BRADY, R. , and GURIN,S. (1951). J. bid. , (in press). BRADY,R. , LUKENS,F. D. , and GURIN,S. Science (in press). BRADY,R. , LUKENS, F. D. , and GURIN,S. (1951b). Unpublished. BUCHANAN, J. , and GURIN,S. (1947). J. bid. , 169, 411. COON, M. J. (1950). J . bid. , 187, 71. ,and QUASTEL, J. H.

Many deuterium-labelled steroids can be prepared by reduction of an unsaturated compound with the hydrogen isotope, and a properly chosen intermediate can furnish a number of compounds for metabolic study. 03 atoms per cent excess deuterium was obtained. This was converted to pregnanolone by known methods and the pregnanolone served as a good intermediate for the preparation of the steroid hormones and metabolites shown in Fig. 1. The products served nicely for tracer experiments since they contained a useful concentration of isotope and their chemical preparation was relatively simple.

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