By Glyn N. Stacey, John G. Day (auth.), John G. Day, Glyn N. Stacey (eds.)
Cryopreservation and Freeze-Drying Protocols: moment variation is a compilation of strong, reproducible thoughts for the conservation of quite a lot of organic fabrics. This generally elevated moment version contains novel ways and protocols for organic fabrics that weren't preservable while the 1st version used to be released in 1995.
This quantity starts with a dialogue of long-term ex situ conservation of organic assets, the position of organic source facilities, and primary ideas of freeze-drying and cryopreservation. every one bankruptcy makes a speciality of the upkeep of particular organic fabrics, together with proteins, mircroorganisms, mobile strains, and multicellular buildings. distinctive descriptions of fabrics required and stepwise protocols are awarded for every upkeep technique, in addition to explanatory notes that spotlight key technical matters. The troubleshooting and history notes supplied for every protocol improve this useful and trustworthy source for the conservation and sustainable use of organic resources.
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Additional resources for Cryopreservation and Freeze-Drying Protocols
15. Franks, F. (1990) Freeze-drying: from empiricism to predictability. Cryoletters 11, 93–110. 16. Franks, F. (1989) Improved freeze-drying: an analysis of the basic scientific principles. Process Biochem. 24, 3–8. 17. Mackenzie, A. P. (1966) Basic principles of freeze-drying for pharmaceuticals. Bull. Parenteral Drug Assoc. 26, 101–129. 18. Cameron, P. (1997) Good Pharmaceutical Freeze-Drying Practice. Interpharm Press, Englewood, CO. 19. Jennings, T. A. (1986) Validation of the lyophilzation process.
W. W. and Morris, G. J. (1987) The Effects of Low Temperatures on Biological Systems. Edward Arnold, London, UK. 42. Farrant, J. (1980) General observations on cell preservation. In: Low Temperature Preservation in Medicine and Biology, (Ashwood-Smith, M. ), Pitman Medical, Tonbridge Wells, UK, pp. 1–18. 43. Ashwood-Smith, M. T. and Farrant, J. (1980) Low Temperature Preservation in Medicine and Biology. Pitman Medical, Tonbridge Wells, UK. 44. , Carpenter, J. , Kita, Y. , and Crowe, J. H. (1990) The basis for toxicity of certain cryoprotectants: a hypothesis.
Ice Structure and Freeze Consolidation (13) A period of consolidation (defined as the hold time), is necessary at the end of sample cooling to ensure that all the vial contents in the sample batch have frozen adequately, although excessive hold times will increase the time of sample freeze and impact on the overall cycle time. It is a fallacy to assume that the ice structure induced remains unchanged during this consolidation period and an ice structure comprising a large number of small ice crystals, induced by rapid cooling, is thermodynamically less stable than an ice structure comprising fewer, larger crystals.
Cryopreservation and Freeze-Drying Protocols by Glyn N. Stacey, John G. Day (auth.), John G. Day, Glyn N. Stacey (eds.)