By Martin Egli, Piet Herdewijn, Akira Matusda
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Extra resources for Current Protocols in Nucleic Acid Chemistry
Ser. 18:69-72. V. 1981. Purine nucleoside synthesis, an efficient method employing nucleoside phosphorylases. Biochemistry 20:3615-3621. , and Vinegar, R. 1986. Imidazo[4,5c]pyridines (3-deazapurines) and their nucleosides as immunosuppresive and antiinflammatory agents. J. Med. Chem. 29:138-143. S. 1952. The enzymically catalysed transfer of the deoxyribosyl group from one purine or pyrimidine to another. Biochem. J. 50:384. P. 1996. Addition of deoxyribose to guanine and modified DNA bases by Lactobacillus helveticus trans-N-deoxyribosylase.
43 mL t-butyl nitrite to the reaction over 5 min while maintaining the bath temperature at −35° to −40°C. Stir the red reaction mixture for 25 min. Work up and purify 2-fluoro-6-O-TMSE-2′-dI 41. Dissolve 23 g K2CO3 in 34 mL water in a 1-liter Erlenmeyer flask. Begin stirring vigorously at 0°C (ice bath). 42. Quench the reaction by slowly pouring the reaction mixture (step 40) into the cold, stirring K2CO3 solution. Rinse the reaction tube with ethyl acetate and add the wash to the neutralized solution.
Elute with a gradient of CH2Cl2/methanol/triethylamine ranging from 95:4:1 to 90:9:1 (v/v/v). 46. 36). 4) should be obtained. The product can be stored indefinitely at –20°C under anhydrous conditions. 3 using a 500-mL round-bottomed flask for the reaction. A mixture of 100 mL anhydrous pyridine and 50 mL toluene is used to dissolve the nucleoside before the addition of the HF/pyridine, which is not diluted before addition at −40°C. 4 should be ∼70%. ALTERNATE PROTOCOL 1 SYNTHESIS OF 2-FLUORO-6-O-(TRIMETHYLSILYLETHYL)-2′DEOXYINOSINE USING 3′,5′-O-DIACETYL-2′-DEOXYGUANOSINE In this procedure, the Mitsunobu reaction can be carried out starting with 3′,5′-O-diacetyl-2′-deoxyguanosine (Fig.
Current Protocols in Nucleic Acid Chemistry by Martin Egli, Piet Herdewijn, Akira Matusda