By T.S. Work and E. Work (Eds.)
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Extra resources for Density Gradient Centrifugation
During manufacture the four tubes connected into the stationary seal are pressed in, and then soldered. This solder has been known to break. It should be remembered that solder is composed of tin and lead, and if samples and gradients come into contact with it, the question of metal contamination should not be ignored. 3. Washing and drying rotors All rotors should be washed with lukewarm water immediately after use and dried. A water wash is usually quite sufficient, but if a detergent must be used then it must not be alkaline.
3 million daltons) is much better resolved by gel electrophoresis than by density gradient centrifugation. 1 million daltons) are almost equally well resolved by the two techniques whereas polysomes are much better resolved by centrifugation than by gel electrophoresis. We would conclude that density gradient centrifugation is not, generally, the best analyricul method for separating particles with a molecular weight of less than one million. However, there are cases where centrifugation may provide a useful method for analysing a mixture of smaller particles.
To avoid accidents all users of centrifuges should be persuaded to read the manuals and other publications referring to care and safety (Price 1972; Burns 1972) as part of a training scheme. It is also useful to have a centrifuge expert in the department who can give advice, train newcomers, do simple troubleshooting and be generally responsible for the machines and rotors. Such a person can often save money by doing simple repairs while also doing a favour to service engineers who hate being called out for such jobs as replacing fuses.
Density Gradient Centrifugation by T.S. Work and E. Work (Eds.)