International Review of Cytology, Vol. 132 - download pdf or read online

By Kwang W. Jeon and Martin Friedlander (Eds.)

ISBN-10: 0123645328

ISBN-13: 9780123645326

This serial provides present advances and accomplished reports in mobile biology - either plant and animal. Articles tackle constructions and keep watch over of gene expression, nucleocytoplasmic interactions, keep watch over of telephone improvement and differentiation, mobile transformation, and development. insurance has been improved to incorporate molecular biology utilized to phone organic platforms, cell-cell interactions, mobilephone floor and receptor phenomena, and lots of different parts. specified topical volumes in components of specific development and curiosity are imminent. those volumes will comprise such subject matters as mitochondrial genomes and the molecular biology of membrane delivery.

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When the synchronized cells, with the sequential treatment with aphidiColin and propyzamide, were treated appropriately with cellulolytic enzymes (as described in Section IV), the protoplasts at the highest MI were obtained (Kakimoto and Shibaoka, 1988). When the enzyme treatment is started 30 min before termination of the propyzamide treatment, protoplasts are prepared 30 min after the release from this treatment. As these protoplasts formed phragmoplast synchronously, the simple disruption of the protoplasts gave phragmoplast-rich fractions, which could now serve for biochemical analysis.

Another important point is that molecular and subcellular studies are easily feasible, as mass culture of TBY-2 cells is readily available. However, there is a laborious point, as this material must be continuously cultured in a proper condition to obtain a high growth rate and high synchrony. This is due to the fact that practical freeze preservation method has not been developed for plant cells. Conse- 28 TOSHlYUKl NAGATA ET AL. quently, this material could accumulate possible point mutations during long-term culture, and so it may not be appropriate for study of the genome structure of plant cells.

S . A . 87,5397-5401. Filner, P. (1965). Exp. Cell Res. 39,33-39. Gamborg, 0. L. (1970). Plant Physiol. 45, 372-375. Gould, A. R. (1984). CRC Crit. Reu. Plant Sci. 4, 315-344. Gunning, B. E. S. (1980). Eur. J . CellBiol. 23, 53-65. Hasezawa, S . , and Nagata. T. (1991). Bot. Aria 104, 206-211. Hasezawa. S . , and Syono, K. (1983). Plant Cell Physiol. 24, 127-132. Hasezawa, S . , and Syono. K. (1988). J . Plant Physiol. 133,46-51. Hasezawa. S.. Hogetsu. T.. and Syono, K. J. Plant Physiol. 134, 115-119.

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International Review of Cytology, Vol. 132 by Kwang W. Jeon and Martin Friedlander (Eds.)


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